Direct measurement of transporter currents in a label-free and real-time manner without using fluorescent probes or radiolabeled ligands.
Currently, the mainstream method for evaluating transporter activity is the uptake assay using RI-labeled substrates. However, there are several issues, such as the need for specialized experimental facilities, the complexity of waste liquid treatment, the fact that the desired substrates are not always commercially available as RI-labeled, and the measurement of endpoints after uptake.
SURFE²R (Surface Electrogenic Event Reader) technology does not require RI labeling, specialized experimental facilities, or waste liquid treatment, and it allows for real-time evaluation of the activity of transporters (symporters, exchangers, unipoters) and pumps.
■ Direct measurement of transporter currents using Solid Supported Membrane (SSM)
■ Experiments can be conducted using membrane fragments prepared from biological membranes or proteoliposomes reconstituted with the target membrane proteins
■ Light stimulation is possible (optional)
■ An all-in-one device that integrates a dispensing machine, measurement section, and computer
■ 150 data points per day
